il 12 beta protein  (MedChemExpress)

Journal: Frontiers in Neurology

Article Title: Elevated interleukin-12B is associated with increased seizure susceptibility: insights from two-sample Mendelian randomization and in vivo experiment

doi: 10.3389/fneur.2025.1706857

Figure Lengend Snippet: Administration of IL-12 beta protein increases seizure susceptibility. (A) Seizure susceptibility measured by 6-Hz seizure threshold test ( n = 12 mice/group). (B) Seizure score within 30 min after picrotoxin administration ( n = 6 mice/group). (C) A representative diagram illustrating EEG, EMG, baseline, and seizure patterns after pilocarpine administration. (D) A time-frequency spectrogram of EEG corresponding to the EEG in (C) ( n = 10 mice/group). (E) Latency to SE after pilocarpine administration. (F) Survival probability within 60 min post pilocarpine administration ( n = 10 mice/group). * p < 0.05, ** p < 0.01 by Student’s t test ( A and E ), generalized linear models with Bonferroni test (B) , Kaplan–Meier analysis with Log Rank (Mantel-Cox) test (F) . Values are means ± SD ( A and E ), medians (IQR) (B) , rate (F) .

Article Snippet: Mice were randomly assigned into two groups. (1) Control group: mice received an intraperitoneal (i.p.) injected of saline (10 mL/kg); (2) IL-12B group: mice were administered an injection of IL-12 beta protein (mouse, HEK293, His; 1 μg/mouse/day, i.p., MedChemExpress, USA) for three consecutive days.

Techniques:

Journal: Molecular Cancer Therapeutics

Article Title: XTX301, a Tumor-Activated Interleukin-12 Has the Potential to Widen the Therapeutic Index of IL12 Treatment for Solid Tumors as Evidenced by Preclinical Studies

doi: 10.1158/1535-7163.MCT-23-0336

Figure Lengend Snippet: Masking of XTX301 activity and reactivation by MMPs in vitro (A) SPR was used to measure the binding kinetics of XTX301, proteolytically cleaved XTX301 (XTX301 + MMP), and rhIL12 to IL12Rβ2. IL12Rβ2 was immobilized to a sensor chip. Then XTX301, proteolytically cleaved XTX301, and rhIL12 were flowed over at concentrations ranging from 3 nmol/L to 400 nmol/L. The concentrations of each analyte decrease from top to bottom within each panel. XTX301 activity was measured in a reporter cell line and primary human cells. B, IL12 HEK-Blue reporter gene cells were incubated with either rhIL12, unmasked control, or XTX301 at varying doses, and the reporter activity was measured. The data represents one of three independent experiments, data points represent the mean of 2 replicate wells and the error bars represent SD. C, Pre-activated primary human PBMCs were incubated with either rhIL12, unmasked control or XTX301 at varying doses, and STAT4 phosphorylation was assessed in CD8 + T cells via flow cytometry. The data points represent the mean of 2 replicate wells and the error bars represent SD. The data represents one of two independent experiments, each conducted with 2 different PBMC donors.

Article Snippet: His-tagged Fc-fused human IL12Rβ2 (R&D systems, catalog no. 1959-B2B) and wild-type recombinant human IL12 (rhIL12; R&D Systems, catalog no.219-IL/CF) were used for surface plasmon resonance (SPR) analysis.

Techniques: Activity Assay, In Vitro, Binding Assay, Incubation, Control, Phospho-proteomics, Flow Cytometry